Human LBP ELISA Kit
Cat no : KE00584
Synonyms
Lipopolysaccharide-binding protein
Validation Data Gallery
Product Information
KE00584 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human LBP ELISA kit is to be used to detect and quantify protein levels of endogenous human LBP. The assay recognizes human LBP. An antibody specific for human LBP has been pre-coated onto the microwells. The human LBP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human LBP is added to detect the captured human LBP protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human LBP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 21.0 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3929 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 89% | 80%-100% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 955.1 | 31.6 | 3.3 |
| 2 | 8 | 245.4 | 8.2 | 3.3 |
| 3 | 8 | 126.3 | 6.2 | 4.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 952.8 | 35.4 | 3.7 |
| 2 | 16 | 237.8 | 11.1 | 4.7 |
| 3 | 16 | 126.5 | 5.9 | 4.7 |
Background Information
LBP (lipopolysaccharide-binding protein) is a serum glycoprotein belonging to the BPI/LBP/Plunc superfamily and BPI/LBP family. It is an acute-phase protein, predominantly synthesized by hepatocytes. LBP is present in normal serum at concentrations of 5 to 10 μg/ml, rising up to 200 μg/ml 24 h after induction of an acute-phase response. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS) which is a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Lipopolysaccharide-binding protein |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.