Human LBR ELISA Kit
Cat no : KE00653
Synonyms
14, 3-beta-hydroxysterol Delta, C-14 sterol reductase, C14SR, Delta(14)-sterol reductase LBR
Validation Data Gallery
Product Information
KE00653 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human LBR ELISA kit is to be used to detect and quantify protein levels of endogenous human LBR. The assay recognizes human LBR. An antibody specific for human LBR has been pre-coated onto the microwells. The human LBR protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human LBR is added to detect the captured human LBR protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human LBR ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.234-15 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3930 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 93% | 90%-95% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 7.53 | 0.60 | 7.96 |
| 2 | 8 | 1.69 | 0.09 | 5.17 |
| 3 | 8 | 0.85 | 0.07 | 8.01 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 7.65 | 0.57 | 7.47 |
| 2 | 16 | 1.71 | 0.09 | 5.12 |
| 3 | 16 | 0.90 | 0.09 | 10.21 |
Background Information
Lamin B Receptor (LBR) is an integral protein of the inner nuclear membrane originally identified by in vitro binding to lamin B. Its N-terminal domain anchors heterochromatin to the nuclear periphery, contributing to the structural integrity of the nucleus and influencing gene expression. Its transmembrane domain acts as a sterol C-14 reductase, which is essential for cholesterol synthesis. LBR mutations are also associated with milder forms of skeletal dysplasia, such as spontaneously regressing bone dysplasia and spondylometaphyseal dysplasia.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 14, 3-beta-hydroxysterol Delta, C-14 sterol reductase, C14SR, Delta(14)-sterol reductase LBR |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.