Speedy™ Human LEPR One-Step ELISA Kit
Cat no : SE50072
Synonyms
CD295, DB, HuB219, LEP R, LEP-R
Validation Data Gallery
Product Information
| Product name | Speedy™ Human LEPR One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 0.10 ng/mL |
| Range | 0.47-30 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3953 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 113% | 95%-120% |
| Cell culture supernatant | 121% | 116%-125% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 15.27 | 0.37 | 2.42 |
| 2 | 8 | 3.84 | 0.08 | 2.08 |
| 3 | 8 | 1.08 | 0.03 | 2.78 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 15.36 | 0.35 | 2.28 |
| 2 | 16 | 3.92 | 0.19 | 4.85 |
| 3 | 16 | 1.08 | 0.03 | 2.78 |
Background Information
The hormone leptin, secreted predominantly from adipose tissue, plays a crucial role in the regulation of numerous neuroendocrine functions, from energy homeostasis to reproduction . Leptin acts through the leptin receptor (LEPR, also known as OB-R), a single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, LEPR mediates signaling through JAK2/STAT3. LEPR plays an important role in the regulation of glucose homeostasis via feeding and adiposity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD295, DB, HuB219, LEP R, LEP-R |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.