Human LIF ELISA Kit
Cat no : KE00689
Synonyms
Leukemia inhibitory factor, Differentiation-stimulating factor, Melanoma-derived LPL inhibitor
Validation Data Gallery
Product Information
KE00689 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human LIF ELISA kit is to be used to detect and quantify protein levels of endogenous human LIF. The assay recognizes human LIF. An antibody specific for human LIF has been pre-coated onto the microwells. The human LIF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human LIF is added to detect the captured human LIF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human LIF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma,Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.3 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3976 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 84% | 70%-114% |
| Cell culture supernatant | 94% | 74%-122% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 532.4 | 26.2 | 4.9 |
| 2 | 8 | 115.7 | 4.3 | 3.7 |
| 3 | 8 | 61.9 | 2.8 | 4.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 529.6 | 19.7 | 3.7 |
| 2 | 16 | 120.3 | 6.1 | 5.0 |
| 3 | 16 | 64.8 | 4.1 | 6.4 |
Background Information
Leukemia Inhibitory Factor (LIF) is a multi-functional cytokine belonging to the interleukin-6 (IL-6) superfamily. Initially identified as a factor that inhibits the proliferation of murine myeloid leukemia cells, LIF has since been found to play important roles in various physiological and pathological processes. These include regulating cell proliferation, maintaining the self-renewal of pluripotent stem cells, tissue/organ development and regeneration, neurogenesis and neural regeneration, maternal reproduction, inflammation, immune response, and metabolism.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Leukemia inhibitory factor, Differentiation-stimulating factor, Melanoma-derived LPL inhibitor |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.