Human LIGHT/TNFSF14 ELISA Kit
Cat no : KE00291
Synonyms
HVEML, CD258, HVEM L, LIGHT, LTg, TNFSF14, TR2
Validation Data Gallery
Product Information
KE00291 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human LIGHT/TNFSF14 ELISA kit is to be used to detect and quantify protein levels of endogenous human LIGHT/TNFSF14. The assay recognizes human LIGHT/TNFSF14. An antibody specific for human LIGHT/TNFSF14 has been pre-coated onto the microwells. The human LIGHT/TNFSF14 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human LIGHT/TNFSF14 is added to detect the captured human LIGHT/TNFSF14 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human LIGHT/TNFSF14 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant，Saliva |
| Assay type | Sandwich |
| Sensitivity | 7.0 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8740 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 92% | 82%-105% |
| Cell culture supernatant | 97% | 73%-129% |
| Saliva | 106% | 90%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,765.0 | 56.5 | 3.2 |
| 2 | 20 | 402.0 | 10.1 | 2.5 |
| 3 | 20 | 105.6 | 4.6 | 4.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,762.8 | 58.1 | 3.3 |
| 2 | 24 | 419.9 | 11.1 | 2.6 |
| 3 | 24 | 106.1 | 5.8 | 5.5 |
Background Information
LIGHT, also known as TNFSF14, HVEML, or CD258, is a type II transmembrane protein that belongs to the TNF superfamily. It is predominantly expressed in lymphoid tissues and produced by activated T cells and immature dendritic cells (DCs). Through its two primary functional receptors HVEM (TNFRSF14) and lymphotoxin β receptor (LTβR), LIGHT signaling is involved in development and maintenance of lymphoid tissues, as well as innate and adaptive immune responses. In addition to the membrane form, LIGHT can also exist as a soluble form generated by cleavage of the extracellular portion of the membrane form.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | HVEML, CD258, HVEM L, LIGHT, LTg, TNFSF14, TR2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.