Human LRRC32 ELISA Kit
Cat no : KE00119
Synonyms
D11S833E, GARP, Garpin, LRRC32
Validation Data Gallery
Product Information
KE00119 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The LRRC32 ELISA kit is to be used to detect and quantify protein levels of endogenous LRRC32. The assay recognizes human LRRC32. An antibody specific for LRRC32 has been pre-coated onto the microwells. The LRRC32 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human LRRC32 is added to detect the captured human LRRC32 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .
| Product name | Human LRRC32 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Tissue lysates |
| Assay type | Sandwich |
| Sensitivity | 0.52 ng/mL |
| Range | 0.94-60 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2615 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human Serum | 85% | 78%-107% |
| Cell culture supernatants | 98% | 88%-117% |
| Tissue lysates | 108% | 86%-122% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 32.67 | 1.66 | 5.1 |
| 2 | 20 | 7.99 | 0.33 | 4.1 |
| 3 | 20 | 2.32 | 0.06 | 2.4 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 32.04 | 2.56 | 8.0 |
| 2 | 24 | 7.59 | 0.67 | 8.8 |
| 3 | 24 | 1.79 | 0.19 | 10.8 |
Background Information
LRRC32, also known as GARP, is a transmembrane protein of 662 amino acids, the extracellular portion of which contains 20 leucine-rich repeats. LRRC32 is a cell surface receptor on regulatory T-lymphocytes (Treg cells), platelets, hepatic stellate cells and certain cancer cells. It has been demonstrated as a Treg-specific activation marker. LRRC32 is critical for the surface expression of latent TGF-β by binding to the complex and functioning as its cell surface receptor. Membrane bound LRRC32 can be shed from cell surface and exists in soluble form.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | D11S833E, GARP, Garpin, LRRC32 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.