Human Lactoferrin/LTF ELISA Kit
Cat no : KE00361
Synonyms
Lactoferrin,LTF,EC:3.4.21.-,GIG 12,GIG12
Validation Data Gallery
Product Information
KE00361 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Lactoferrin/LTF ELISA kit is to be used to detect and quantify protein levels of endogenous human Lactoferrin/LTF. The assay recognizes human Lactoferrin/LTF. An antibody specific for human Lactoferrin/LTF has been pre-coated onto the microwells. The human Lactoferrin/LTF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Lactoferrin/LTF is added to detect the captured human Lactoferrin/LTF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Lactoferrin/LTF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine, Saliva |
| Assay type | Sandwich |
| Sensitivity | 5.8 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4057 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Serum | 96% | 86%-108% |
| Urine | 93% | 74%-119% |
| Saliva | 79% | 71%-88% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 464.0 | 25.8 | 5.6 |
| 2 | 20 | 109.7 | 3.6 | 3.2 |
| 3 | 20 | 28.4 | 1.5 | 5.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 475.7 | 29.7 | 6.3 |
| 2 | 24 | 115.6 | 6.5 | 5.6 |
| 3 | 24 | 32.3 | 3.2 | 9.8 |
Background Information
Lactoferrin (LTF), known as an iron-binding glycoprotein, is the most abundant element in milk. Prior research has illustrated that LTFbears multi-pharmacological properties, including protection against infection, regulation inflammatory response, antioxidant and antifibrotic process. Moreover, clinical trials have identified the safety of oral administration of bovine LTF and demonstrated the efficacy of LTF on anti-infection, treatment of inflammation, cancer, metabolic disorders.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Lactoferrin,LTF,EC:3.4.21.-,GIG 12,GIG12 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.