Human MAGEA3 ELISA Kit
Cat no : KE00701
Synonyms
Antigen MZ2 D, Antigen MZ2-D, Cancer/testis antigen 1.3, CT1.3, HIP8
Validation Data Gallery
Product Information
KE00701 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MAGEA3 ELISA kit is to be used to detect and quantify protein levels of endogenous human MAGEA3. The assay recognizes human MAGEA3. An antibody specific for human MAGEA3 has been pre-coated onto the microwells. The human MAGEA3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MAGEA3 is added to detect the captured human MAGEA3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MAGEA3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 8.7 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4102 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 107% | 105%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,074.4 | 33.1 | 3.1 |
| 2 | 8 | 395.5 | 21.9 | 5.5 |
| 3 | 8 | 206.5 | 17.6 | 8.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,144.0 | 81.3 | 7.1 |
| 2 | 16 | 387.5 | 26.6 | 6.9 |
| 3 | 16 | 203.2 | 19.0 | 9.3 |
Background Information
MAGEA3 is a cancer testis antigen that is typically expressed in various cancers but not in normal adult tissues except testis and placenta. It has been identified as a potential biomarker and therapeutic target for cancer immunotherapy. Studies have shown that MAGEA3 is expressed in non-small cell lung cancer and is associated with poor prognosis. Additionally, MAGEA3 expression has been detected in other cancers such as breast cancer and gastric cancer. Its expression is often regulated by epigenetic mechanisms. MAGEA3 has also been explored as a target for cancer vaccines and T-cell therapies.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Antigen MZ2 D, Antigen MZ2-D, Cancer/testis antigen 1.3, CT1.3, HIP8 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.