Human MCM2 ELISA Kit
Cat no : KE00483
Synonyms
MCM2,BM28,CCNL1,CDCL1,EC:3.6.4.12
Validation Data Gallery
Product Information
KE00483 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MCM2 ELISA kit is to be used to detect and quantify protein levels of endogenous human MCM2. The assay recognizes human MCM2. An antibody specific for human MCM2 has been pre-coated onto the microwells. The human MCM2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MCM2 is added to detect the captured human MCM2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MCM2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 11.6 pg/mL |
| Range | 93.8-6000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4171 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 85% | 83%-86% |
| Cell lysate | 95% | 86%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,619.5 | 107.2 | 4.1 |
| 2 | 8 | 678.2 | 28.4 | 4.2 |
| 3 | 8 | 320.2 | 19.9 | 6.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,498.3 | 163.2 | 6.5 |
| 2 | 16 | 624.7 | 61.3 | 9.8 |
| 3 | 16 | 309.7 | 19.2 | 6.2 |
Background Information
MCM2 (microchromosome maintenance protein 2) is an important member of the family of microchromosome maintenance proteins (MCM family of proteins) involved in the initiation and elongation of DNA replication in eukaryotic cells. MCM2, together with MCM3-MCM7, forms the hexameric MCM2-7 complex, which serves as a replicative deconjugating enzyme that utilizes the energy of ATP hydrolysis to drive DNA deconjugation, ensuring that DNA is replicated only once per cell cycle. MCM2 is loaded onto chromatin during the G1 phase of the cell cycle and is a marker for S-phase cells. Its expression level is closely related to cell proliferation and is up-regulated in a variety of tumors (e.g. breast cancer, colorectal cancer, lung cancer, etc.), and can be used as a marker for precancerous lesions and tumor progression.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MCM2,BM28,CCNL1,CDCL1,EC:3.6.4.12 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.