Human MCM3 ELISA Kit
Cat no : KE00821
Synonyms
DNA polymerase alpha holoenzyme-associated protein P1, EC:3.6.4.12, P1 MCM3, p102, P1-MCM3
Validation Data Gallery
Product Information
KE00821 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MCM3 ELISA kit is to be used to detect and quantify protein levels of endogenous human MCM3. The assay recognizes human MCM3. An antibody specific for human MCM3 has been pre-coated onto the microwells. The human MCM3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MCM3 is added to detect the captured human MCM3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MCM3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.2-12.5 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4172 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 101% | 90%-122% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 6.39 | 0.27 | 4.29 |
| 2 | 8 | 1.50 | 0.09 | 5.99 |
| 3 | 8 | 0.68 | 0.03 | 4.30 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.90 | 0.56 | 9.44 |
| 2 | 16 | 1.39 | 0.14 | 9.79 |
| 3 | 16 | 0.66 | 0.05 | 6.93 |
Background Information
Minichromosome maintenance protein 3 (MCM3) is a key component of the MCM protein family, which plays a crucial role in the initiation and regulation of DNA replication. MCM3 is highly conserved across species and is essential for maintaining genomic stability. In recent years, research has shown that MCM3 is overexpressed in various cancers, including hepatocellular carcinoma (HCC), ovarian cancer, and medulloblastoma. MCM3 is involved in multiple biological processes, such as cell cycle regulation and cell proliferation. Functional enrichment analysis suggests that MCM3 is mainly involved in cell cycle and cell metabolic pathways. Furthermore, MCM3 is involved in the formation of pre-replicative complexes and is regulated by phosphorylation during the cell cycle.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | DNA polymerase alpha holoenzyme-associated protein P1, EC:3.6.4.12, P1 MCM3, p102, P1-MCM3 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.