Human MCP-1/CCL2 ELISA Kit
Cat no : KE00425
Synonyms
CCL2,MCP1,MCP-1,C C motif chemokine 2,C-C motif ch
Validation Data Gallery
Product Information
KE00425 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MCP-1/CCL2 ELISA kit is to be used to detect and quantify protein levels of endogenous human MCP-1/CCL2. The assay recognizes human MCP-1/CCL2. An antibody specific for human MCP-1/CCL2 has been pre-coated onto the microwells. The human MCP-1/CCL2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MCP-1/CCL2 is added to detect the captured human MCP-1/CCL2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MCP-1/CCL2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine |
| Assay type | Sandwich |
| Sensitivity | 0.5 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6347 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 95% | 89%-102% |
| Cell culture supernatant | 102% | 98%-107% |
| Urine | 92% | 89%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 272.4 | 12.5 | 4.6 |
| 2 | 20 | 61.4 | 0.6 | 1.0 |
| 3 | 20 | 14.9 | 0.3 | 2.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 274.0 | 17.6 | 6.4 |
| 2 | 24 | 61.8 | 2.6 | 4.3 |
| 3 | 24 | 15.7 | 0.4 | 2.6 |
Background Information
Monocyte chemotactic protein 1 (MCP1; also known as CCL2), is a chemokine that can be expressed in monocytes, macrophages, and endothelial cells, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. Research has shown that the expression of MCP1 increases in the serum of patients with acute myocarditis. MCP1 is up-regulated in many types of CNS injury, including ischemia, hemorrhage, trauma, infection, hypoxia, and peripheral nerve axotomy. MCP1 has also been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CCL2,MCP1,MCP-1,C C motif chemokine 2,C-C motif ch |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Theranostics Discovery of indole analogue Tc3 as a potent pyroptosis inducer and identification of its combination strategy against hepatic carcinoma | ||
Sci Rep Mechanistic studies of Ca2+-induced classical pyroptosis pathway promoting renal adhesion on calcium oxalate kidney stone formation | ||
Cell Death Dis Single-cell landscape identified SERPINB9 as a key player contributing to stemness and metastasis in non-seminomas |