Human MCT2/SLC16A7 ELISA Kit
Cat no : KE00875
Synonyms
SLC16A7, MCT 2, MCT2, Monocarboxylate transporter 2, Solute carrier family 16 member 7
Validation Data Gallery
Product Information
KE00875 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MCT2/SLC16A7 ELISA kit is to be used to detect and quantify protein levels of endogenous human MCT2/SLC16A7. The assay recognizes human MCT2/SLC16A7. An antibody specific for human MCT2/SLC16A7 has been pre-coated onto the microwells. The human MCT2/SLC16A7 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MCT2/SLC16A7 is added to detect the captured human MCT2/SLC16A7 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MCT2/SLC16A7 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 8.6 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9194 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 107% | 104%-110% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,210.1 | 41.0 | 3.4 |
| 2 | 8 | 400.4 | 2.4 | 0.6 |
| 3 | 8 | 158.2 | 2.4 | 1.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,209.7 | 30.5 | 2.5 |
| 2 | 16 | 397.0 | 5.4 | 1.4 |
| 3 | 16 | 157.5 | 2.6 | 1.7 |
Background Information
SLC16A7, also named MCT2, is a proton-coupled monocarboxylate transporter. MCT2/SLC16A7 has the highest affinity for pyruvate and lactate among all the functionally characterized MCTs, serving as a primary pyruvate transporter in humans. In human cancers, MCT2 has been described as strongly expressed in the cytoplasm of colorectal cancer cells, indicating a possible role in intracellular organelles, such as mitochondria.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | SLC16A7, MCT 2, MCT2, Monocarboxylate transporter 2, Solute carrier family 16 member 7 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.