Human MDC1 ELISA Kit
Cat no : KE00493
Synonyms
MDC1,MDC 1,NFBD 1,NFBD1
Validation Data Gallery
Product Information
KE00493 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MDC1 ELISA kit is to be used to detect and quantify protein levels of endogenous human MDC1. The assay recognizes human MDC1. An antibody specific for human MDC1 has been pre-coated onto the microwells. The human MDC1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MDC1 is added to detect the captured human MDC1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MDC1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 2.3 pg/mL |
| Range | 19.5-1250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9656 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 103% | 98%-109% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 575.0 | 54.9 | 9.6 |
| 2 | 8 | 128.0 | 4.3 | 3.4 |
| 3 | 8 | 70.9 | 4.3 | 6.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 569.4 | 53.4 | 9.4 |
| 2 | 16 | 137.3 | 10.8 | 7.9 |
| 3 | 16 | 73.4 | 5.4 | 7.3 |
Background Information
The DNA damage response (DDR) is crucial for the maintenance of genome integrity and cell survival.Mediator of DNA damage checkpoint 1 (MDC1), also known as nuclear factor with BRCT domains 1 (NFBD1), is a scaffold protein involved in the early steps of the DDR, accumulating at sites of DNA double-stranded breaks within one minute of genomic insult.MDC1 functions to amplify the phosphorylation of H2AX to form γ-H2AX. γ-H2AX can extend as much as 2 Mb beyond the location of the DNA double-stranded break. MDC1 also interacts directly with p53 to inhibit its activity and prevent apoptosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MDC1,MDC 1,NFBD 1,NFBD1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.