Human MDM1 ELISA Kit
Cat no : KE00405
Synonyms
MDM1,Nuclear protein MDM1
Validation Data Gallery
Product Information
KE00405 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MDM1 ELISA kit is to be used to detect and quantify protein levels of endogenous human MDM1. The assay recognizes human MDM1. An antibody specific for human MDM1 has been pre-coated onto the microwells. The human MDM1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MDM1 is added to detect the captured human MDM1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MDM1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 12.0 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 56890 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 95% | 90%-99% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,748.3 | 84.3 | 4.8 |
| 2 | 20 | 401.1 | 8.2 | 2.0 |
| 3 | 20 | 129.5 | 4.5 | 3.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,742.7 | 101.7 | 5.8 |
| 2 | 24 | 380.1 | 17.5 | 4.6 |
| 3 | 24 | 121.0 | 5.5 | 4.5 |
Background Information
Mdm1 nuclear protein (MDM1) is a microtubule-binding nuclear protein that localizes to the centrioles of dividing cells and differentiating multiciliated cells. It plays a crucial role in negatively regulating centriole duplication. MDM1 may also play a significant role in the function and structure of photoreceptor cells in the retina. Its dysfunction may be related to neurodevelopmental defects, polycystic kidneys, obesity, polydactyly, and retinal degeneration. The soluble form of MDM1 or its functional equivalents may participate in cellular processes beyond the nucleus and centrioles, potentially influencing endolysosomal dynamics and lipid homeostasis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MDM1,Nuclear protein MDM1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.