Human MDMX/MDM4 ELISA Kit
Cat no : KE00795
Synonyms
MDM4, MDMX, HDMX, Mdm2-like p53-binding protein, p53-binding protein Mdm4
Validation Data Gallery
Product Information
KE00795 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MDMX/MDM4 ELISA kit is to be used to detect and quantify protein levels of endogenous human MDMX/MDM4. The assay recognizes human MDMX/MDM4. An antibody specific for human MDMX/MDM4 has been pre-coated onto the microwells. The human MDMX/MDM4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MDMX/MDM4 is added to detect the captured human MDMX/MDM4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MDMX/MDM4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 2.6 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4194 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 97% | 95%-100% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,065.3 | 28.1 | 2.6 |
| 2 | 8 | 274.2 | 8.1 | 3.0 |
| 3 | 8 | 79.5 | 2.2 | 2.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,066.3 | 32.9 | 3.1 |
| 2 | 16 | 276.9 | 7.4 | 2.7 |
| 3 | 16 | 80.9 | 3.2 | 3.9 |
Background Information
MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2, thereby preventing p53-mediated cell cycle arrest and apoptosis. MDM4 resides mostly in the cytoplasm but can be recruited to the nucleus by MDM2.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MDM4, MDMX, HDMX, Mdm2-like p53-binding protein, p53-binding protein Mdm4 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.