Human MICA ELISA Kit
Cat no : KE00925
Synonyms
MIC A, MIC-A
Validation Data Gallery
Product Information
KE00925 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MICA ELISA kit is to be used to detect and quantify protein levels of endogenous human MICA. The assay recognizes human MICA. An antibody specific for human MICA has been pre-coated onto the microwells. The human MICA protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MICA is added to detect the captured human MICA protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MICA ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 1.6 pg/mL |
| Range | 23.4-1500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 100507436 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 94% | 86%-101% |
| Cell culture supernatant | 85% | 80%-89% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 805.5 | 44.5 | 5.5 |
| 2 | 8 | 154.7 | 11.2 | 7.3 |
| 3 | 8 | 92.3 | 9.0 | 9.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 817.7 | 37.1 | 4.5 |
| 2 | 16 | 172.2 | 21.4 | 12.4 |
| 3 | 16 | 90.4 | 7.6 | 8.5 |
Background Information
MICA (MHC class I chain-related A) protein is a stress-induced molecule expressed on epithelial cells and fibroblasts. It activates NK cells and T cells via the NKG2D receptor, playing a vital role in immune surveillance against cancer and infections. MICA is also implicated in autoimmune diseases and transplant rejection. Its polymorphisms can affect immune responses and disease susceptibility.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MIC A, MIC-A |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.