Human MKNK2 ELISA Kit
Cat no : KE00555
Synonyms
MKNK2, EC:2.7.11.1, GPRK7, MAP kinase signal-integrating kinase 2, MAP kinase-interacting serine/threonine-protein kinase 2
Validation Data Gallery
Product Information
KE00555 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MKNK2 ELISA kit is to be used to detect and quantify protein levels of endogenous human MKNK2. The assay recognizes human MKNK2. An antibody specific for human MKNK2 has been pre-coated onto the microwells. The human MKNK2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MKNK2 is added to detect the captured human MKNK2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MKNK2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2872 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 99% | 93%-105% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 12.56 | 1.08 | 8.58 |
| 2 | 8 | 2.37 | 0.15 | 6.41 |
| 3 | 8 | 0.74 | 0.04 | 5.92 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 12.04 | 1.09 | 9.03 |
| 2 | 16 | 2.54 | 0.23 | 9.07 |
| 3 | 16 | 0.76 | 0.05 | 6.74 |
Background Information
MKNK2 (MAP kinase-interacting serine/threonine-protein kinase 2) is also named as GPRK7, MNK2 and belongs to the CAMK Ser/Thr protein kinase family. It has a conserved C-terminal Erk-interacting domain, a catalytic domain with homology to the calcium/calmodulin-dependent family of kinases, and putative MAP kinase phosphorylation sites located within the T loop of the kinase domain. MKNK2 was reported to function as an oncogene. MKNK2 over-expression promoted the development prostate cancer and glioma through mediating various cellular processes, such as the classic translation/proliferation signals, cell growth and proliferation, as well as invasion.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MKNK2, EC:2.7.11.1, GPRK7, MAP kinase signal-integrating kinase 2, MAP kinase-interacting serine/threonine-protein kinase 2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.