Human MLL4 ELISA Kit
Cat no : KE00905
Synonyms
CXXC10 , DYT28, HRX2, KIAA0304, KMT2B
Validation Data Gallery
Product Information
KE00905 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MLL4 ELISA kit is to be used to detect and quantify protein levels of endogenous human MLL4. The assay recognizes human MLL4. An antibody specific for human MLL4 has been pre-coated onto the microwells. The human MLL4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MLL4 is added to detect the captured human MLL4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MLL4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 16.8 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9757 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 103% | 88%-109% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,716.7 | 48.5 | 2.8 |
| 2 | 8 | 424.0 | 27.5 | 6.5 |
| 3 | 8 | 218.5 | 3.4 | 1.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,758.2 | 64.5 | 3.7 |
| 2 | 16 | 430.2 | 20.7 | 4.8 |
| 3 | 16 | 226.8 | 10.0 | 4.4 |
Background Information
MLL4, also known as KMT2B, which is an essential subunit of the H3K4 methylation complexes, regulates cell type-specific transcriptional programs through enhancer activation. Taspase 1 mediates cleavage of the MLL protein, generating 320 kDa N-terminal (MLL-N) and 180 kDa C-terminal (MLL-C) fragments.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CXXC10 , DYT28, HRX2, KIAA0304, KMT2B |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.