Human MLLT1 ELISA Kit
Cat no : KE00439
Synonyms
MLLT1,ENL,LTG19,MLLT 1,Protein ENL
Validation Data Gallery
Product Information
KE00439 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MLLT1 ELISA kit is to be used to detect and quantify protein levels of endogenous human MLLT1. The assay recognizes human MLLT1. An antibody specific for human MLLT1 has been pre-coated onto the microwells. The human MLLT1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MLLT1 is added to detect the captured human MLLT1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MLLT1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 1.9 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4298 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 96% | 93%-100% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 963.0 | 21.7 | 2.3 |
| 2 | 8 | 243.7 | 11.9 | 4.9 |
| 3 | 8 | 75.3 | 3.4 | 4.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 961.4 | 44.1 | 4.6 |
| 2 | 16 | 239.5 | 11.5 | 4.8 |
| 3 | 16 | 78.3 | 4.9 | 6.2 |
Background Information
MLLT1 (also known as ENL, YEATS1) is a protein that plays a significant role in transcriptional regulation and epigenetic processes. MLLT1 contains a conserved YEATS domain at its N-terminus, which is crucial for its function as an epigenetic reader. The C-terminal region of MLLT1 includes an ANC1 homology domain, which is involved in protein-protein interactions with components of the Super Elongation Complex (SEC) and DOT1L complex. MLLT1 is a multifunctional protein with critical roles in transcriptional regulation and epigenetic processes. Its involvement in leukemia and other cancers makes it an important target for therapeutic development.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MLLT1,ENL,LTG19,MLLT 1,Protein ENL |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.