Human MMP-1 Antibody Pair Set
Cat no : PA00151
Synonyms
Validation Data Gallery
Product Information
| Product name | Human MMP-1 Antibody Pair Set |
| Tests | 10 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | |
| Range | 19.5-1250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4312 |
Recovery
| Sample Type | Average | Range |
|---|
IntraAssay
| Sample | n | mean () | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? | ? | ? |
| 2 | 20 | ? | ? | ? |
| 3 | 20 | ? | ? | ? |
InterAssay
| Sample | n | mean () | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | ? | ? | ? |
| 2 | 24 | ? | ? | ? |
| 3 | 24 | ? | ? | ? |
Background Information
Matrix metalloproteinase 1 (MMP1), which is also known as interstitial collagenase and fibroblast collagenase is a member of the matrix metalloproteinases (MMPs) family . Proteins in this family mainly participate in the breakdown of extracellular matrix both in normal physiological processes and disease processes. Several factors contribute to the expression of MMP1, including endogenous factors, such as polymorphisms and epigenetic regulation in the promoter region of MMP1, as well as exogenous factors, such as the tumor microenvironment. A variety of MMP1 promoter genotypic polymorphisms have been reported in various ethnic populations, and their contributions to different disease risks have been explored. In the tumor microenvironment, a variety of inflammatory factors, including interleukin-8 (IL-8), IL-1β and tumor necrosis factor-α (TNF-α), have been reported to induce MMP1 expression in cancer cells. (PMID: 31115512, PMID: 28334049)
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.