Human MMP-9 ELISA Kit
Cat no : KE00298
Synonyms
MMP9, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, CLG4B, EC:3.4.24.35
Validation Data Gallery
Product Information
KE00298 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MMP-9 ELISA kit is to be used to detect and quantify protein levels of endogenous MMP-9. The assay recognizes human MMP-9. An antibody specific for MMP-9 has been pre-coated onto the microwells. The MMP-9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for MMP-9 is added to detect the captured MMP-9 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MMP-9 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Saliva, Urine |
| Assay type | Sandwich |
| Sensitivity | 16.6 pg/mL |
| Range | 125-8000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4318 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human Serum | 85% | 79%-91% |
| Cell culture supernatant | 92% | 86%-100% |
| Saliva | 91% | 75%-107% |
| Urine | 85% | 81%-87% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 3,875.1 | 199.6 | 5.2 |
| 2 | 20 | 957.3 | 26.1 | 2.7 |
| 3 | 20 | 250.3 | 5.4 | 2.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 3,831.8 | 137.2 | 3.6 |
| 2 | 24 | 917.2 | 28.5 | 3.1 |
| 3 | 24 | 241.6 | 8.5 | 3.5 |
Background Information
MMP9 (matrix metallopeptidase 9), also named as Gelatinase B, is a member of matrix metalloproteinase (MMP) family. The MMP family of enzymes is comprised of critically important extracellular matrix remodeling proteases whose activity has been implicated in normal embryogenesis, tissue remodelling and many diseases such as arthritis, cancer, periodontitis, glomerulonephritis, encephalomyelitis, atherosclerosis and tissue ulceration. These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP9 is produced by a variety of normal and transformed cells including neutrophils, monocytes, macrophages, astrocytes, fbroblasts, osteoclasts and so on. In tumors, MMP-9 destroys collagen (type IV) in the vascular basal membrane in the vicinity of tumor cells which invade the surrounding tissues and contributes to metastasis. Circulating levels of MMP-9 are increased in many inflammatory disorders such as atherosclerosis, hepatitis C virus infection and colorectal cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MMP9, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, CLG4B, EC:3.4.24.35 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Oral Dis Evidence of microbiota-host dysbiosis between periodontitis and cerebral small vessel disease | ||
Sci Rep The association of defective pleural sRAGE production with the recurrence of malignant pleural effusion after Talc pleurodesis. | ||
Bioact Mater In vivo circRNA-engineered macrophages mediate localized MMP9 neutralization to rejuvenate aged bone. |