Human MMP-8 ELISA Kit
Cat no : KE00218
Synonyms
MMP8, CLG1, EC:3.4.24.34, HNC, Matrix metalloproteinase 8
Validation Data Gallery
Product Information
KE00218 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MMP-8 ELISA kit is to be used to detect and quantify protein levels of endogenous MMP-8. The assay recognizes human MMP-8. An antibody specific for MMP-8 has been pre-coated onto the microwells. The MMP-8 protein in samples is captured by the coated antibody after incubation.Following extensive washing, another antibody of biotinylated specific for MMP-8 is added to detect the captured MMP-8 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MMP-8 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Tissue lysates, Saliva |
| Assay type | Sandwich |
| Sensitivity | 17.4 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4317 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 105% | 90%-113% |
| cell culture supernatants | 108% | 84%-129% |
| saliva | 107% | 95%-121% |
| Tissue Lysates | 85% | 76%-97% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 416.3 | 10.3 | 2.5 |
| 2 | 20 | 638.2 | 16.9 | 2.7 |
| 3 | 20 | 2,013.3 | 110.7 | 5.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 348.4 | 9.4 | 2.7 |
| 2 | 24 | 594.0 | 20.8 | 3.5 |
| 3 | 24 | 2,026.5 | 107.3 | 5.3 |
Background Information
MMP-8(Matrix metalloproteinase-8) an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells(PMID:15367487). The high-serum MMP-8 levels (>100 ng/mL) had poor cancer-specific survival(PMID: 29808017). MMP8 also has prognosis in patients with gastric cancer(PMID: 30192205).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MMP8, CLG1, EC:3.4.24.34, HNC, Matrix metalloproteinase 8 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.