Human MPO ELISA Kit
Cat no : KE00353
Synonyms
MPO,EC:1.11.2.2,myeloperoxidase
Validation Data Gallery
Product Information
KE00353 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MPO ELISA kit is to be used to detect and quantify protein levels of endogenous human MPO. The assay recognizes human MPO. An antibody specific for human MPO has been pre-coated onto the microwells. The human MPO protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MPO is added to detect the captured human MPO protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MPO ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Saliva, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 10.1 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4353 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 86% | 77%-93% |
| Cell culture supernatant | 81% | 71%-98% |
| Urine | 79% | 72%-92% |
| Saliva | 100% | 79%-120% |
| Cell lysate | 96% | 72%-114% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,309.5 | 67.3 | 5.1 |
| 2 | 20 | 307.7 | 15.5 | 5.0 |
| 3 | 20 | 70.7 | 4.5 | 6.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,267.8 | 35.5 | 2.8 |
| 2 | 24 | 299.5 | 20.8 | 6.9 |
| 3 | 24 | 70.6 | 5.9 | 8.3 |
Background Information
MPO(myeloperoxidase) is a peroxidase enzyme presented in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. Plasma concentration of MPO can be used as a specific marker of PMN activation. MPO catalyzes the production of hypochlorous acid (HClO) from hydrogen peroxide (H2O2) and chloride anion (Cl-, or the equivalent from a non-chlorine halide). This enzymatic system plays an important role in human defense against microorganisms. The serum/plasma MPO levels have been associated with a variety of clinical conditions including inflammatory diseases, atherosclerosis, ischaemic stroke, hypertension, heart failure, risk of cardiovascular events and so on.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MPO,EC:1.11.2.2,myeloperoxidase |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Front Immunol Plasma inflammatory cytokines are associated with cognitive impairment after acute minor ischemic stroke and transient ischemic attack | ||
Front Med (Lausanne) Evaluation of neutrophil activation in autoimmune kidney diseases using blood heparin-binding protein: a pilot study. | ||
J Forensic Leg Med The mechanism of serum cell-free DNA release in postmortem subjects and novel markers to estimate the postmortem intervals | ||
Cell Rep SARS-CoV-2 SUD2core promotes neutrophil-mediated inflammation by disabling HEBP2-mediated restraint on granule exocytosis. |