Human MRP3/ABCC3 ELISA Kit
Cat no : KE00616
Synonyms
ABCC3, MRP3, ABC31, ATP-binding cassette sub-family C member 3, EC:7.6.2.-
Validation Data Gallery
Product Information
KE00616 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MRP3/ABCC3 ELISA kit is to be used to detect and quantify protein levels of endogenous human MRP3/ABCC3. The assay recognizes human MRP3/ABCC3. An antibody specific for human MRP3/ABCC3 has been pre-coated onto the microwells. The human MRP3/ABCC3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MRP3/ABCC3 is added to detect the captured human MRP3/ABCC3 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MRP3/ABCC3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 13.8 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8714 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 83% | 76%-97% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,162.1 | 112.2 | 5.2 |
| 2 | 8 | 554.9 | 19.5 | 3.5 |
| 3 | 8 | 288.6 | 10.2 | 3.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,032.0 | 183.3 | 9.0 |
| 2 | 16 | 513.9 | 54.3 | 10.6 |
| 3 | 16 | 272.6 | 26.3 | 9.6 |
Background Information
MRP3 also known as ABCC3, belongs to ATP-binding cassette (ABC) transporter protein superfamily. MRP3 hydrolyzes ATP to enable active transport of various substrates including many drugs, toxicants, and endogenous compounds across cell membranes. In normal liver, MRP3 was found present mainly in the bile ducts. In livers of patients with various forms of cholestasis, MRP3 levels were frequently increased in the proliferative cholangiocytes, suggesting its role in cholehepatic and enterohepatic bile circulation and in protecting liver from toxic bile salts.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ABCC3, MRP3, ABC31, ATP-binding cassette sub-family C member 3, EC:7.6.2.- |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.