Human MSLN ELISA Kit
Cat no : KE00383
Synonyms
Mesothelin,MSLN,CAK1,CAK1 antigen,Megakaryocyte-po
Validation Data Gallery
Product Information
KE00383 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MSLN ELISA kit is to be used to detect and quantify protein levels of endogenous human MSLN. The assay recognizes human MSLN. An antibody specific for human MSLN has been pre-coated onto the microwells. The human MSLN protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MSLN is added to detect the captured human MSLN protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MSLN ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Saliva |
| Assay type | Sandwich |
| Sensitivity | 3.7 pg/mL |
| Range | 39.1-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10232 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Urine | 92% | 88%-95% |
| Saliva | 93% | 91%-95% |
| Cell cultue supernatant | 90% | 89%-91% |
| Human serum | 98% | 92%-107% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,291.8 | 69.7 | 5.4 |
| 2 | 20 | 281.7 | 13.7 | 4.9 |
| 3 | 20 | 69.4 | 3.3 | 4.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,232.9 | 45.8 | 3.7 |
| 2 | 24 | 275.7 | 9.4 | 3.4 |
| 3 | 24 | 72.1 | 2.6 | 3.6 |
Background Information
Mesothelin (MSLN) is a glycosylphosphatidylinositol-linked membrane glycoprotein which is highly expressed in a variety of tumors and is also expressed in mesothelial cells of healthy individuals, but at low levels. Therefore, it can be considered as a promising target protein for tumor-targeted therapy. In the tumor environment, MSLN plays an important role in survival, proliferation, and migration/invasion of cancer cells as well as in drug resistance. Over the years, evidence has accumulated with regards to the importance of MSLN as a tumor-associated antigen (TAA) overexpressed in almost one-third of human cancers. (PMID: 35795680)
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Mesothelin,MSLN,CAK1,CAK1 antigen,Megakaryocyte-po |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.