Human MT-ND5 ELISA Kit
Cat no : KE00516
Synonyms
MT-ND5, ND5, MT ND5, MTND5, NADH5
Validation Data Gallery
Product Information
KE00516 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MT-ND5 ELISA kit is to be used to detect and quantify protein levels of endogenous human MT-ND5. The assay recognizes human MT-ND5. An antibody specific for human MT-ND5 has been pre-coated onto the microwells. The human MT-ND5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MT-ND5 is added to detect the captured human MT-ND5 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MT-ND5 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.31 ng/mL |
| Range | 3.13-200 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4540 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 80% | 73%-88% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 116.39 | 5.36 | 4.60 |
| 2 | 8 | 34.46 | 1.94 | 5.62 |
| 3 | 8 | 10.10 | 0.26 | 2.62 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 115.34 | 5.86 | 5.08 |
| 2 | 16 | 34.58 | 1.59 | 4.61 |
| 3 | 16 | 10.13 | 0.57 | 5.63 |
Background Information
MT-ND5 is a mitochondrial gene that encodes a core subunit of Complex I (NADH:ubiquinone oxidoreductase), the largest enzyme in the mitochondrial electron transport chain (ETC). Complex I is essential for cellular respiration, as it initiates the process by transferring electrons from NADH to ubiquinone (coenzyme Q), coupled with the translocation of protons across the mitochondrial inner membrane to generate the proton gradient required for ATP synthesis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MT-ND5, ND5, MT ND5, MTND5, NADH5 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.