Human MTNR1A ELISA Kit
Cat no : KE00770
Synonyms
MEL 1A R, Mel1a receptor, Mel-1A-R, melatonin receptor 1A, Melatonin receptor type 1A
Validation Data Gallery
Product Information
KE00770 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MTNR1A ELISA kit is to be used to detect and quantify protein levels of endogenous human MTNR1A. The assay recognizes human MTNR1A. An antibody specific for human MTNR1A has been pre-coated onto the microwells. The human MTNR1A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MTNR1A is added to detect the captured human MTNR1A protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MTNR1A ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.11 ng/mL |
| Range | 0.243-15 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4543 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 98% | 94%-103% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 6.93 | 0.18 | 2.62 |
| 2 | 8 | 1.79 | 0.07 | 3.70 |
| 3 | 8 | 0.92 | 0.03 | 2.99 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 7.51 | 0.69 | 9.24 |
| 2 | 16 | 1.86 | 0.12 | 6.28 |
| 3 | 16 | 0.93 | 0.05 | 5.57 |
Background Information
MTNR1A (melatonin 1A receptor) is mainly distributed in the suprachiasmatic nucleus and pituitary nodules in the hypothalamus of mammals, which is related to the regulation of animal reproduction. Melatonin (MT) can specifically bind to MTNR1A on the cell membrane and regulate mammalian reproductive activities.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MEL 1A R, Mel1a receptor, Mel-1A-R, melatonin receptor 1A, Melatonin receptor type 1A |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.