Human Mammaglobin A ELISA Kit
Cat no : KE00071
Synonyms
Mammaglobin 1, Mammaglobin A, MGB1, SCGB2A2, UGB2
Validation Data Gallery
Product Information
KE00071 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mammaglobin A ELISA kit is to be used to detect and quantify protein levels of endogenous Mammaglobin A. The assay recognizes human Mammaglobin A. An antibody specific for Mammaglobin A has been pre-coated onto the microwells. The Mammaglobin A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for Mammaglobin A is added to detect the captured Mammaglobin A protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Mammaglobin A ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine |
| Assay type | Sandwich |
| Sensitivity | 5.0 pg/mL |
| Range | 125-8000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4250 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Urine | 94% | 85%-113% |
| Human plasma | 103% | 92%-111% |
| Cell culture supernatants | 104% | 86%-125% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 6,124.8 | 545.7 | 8.9 |
| 2 | 20 | 1,593.5 | 105.5 | 6.6 |
| 3 | 20 | 343.8 | 20.1 | 5.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 5,411.6 | 483.5 | 8.9 |
| 2 | 24 | 2,572.7 | 133.4 | 5.2 |
| 3 | 24 | 485.1 | 22.9 | 4.7 |
Background Information
Mammaglobin A, also known as SCGB2A2, is a member of the secretoglobin superfamily. Mammaglobin A is expressed in normal mammary gland epithelium and is over-expressed in primary and metastatic human breast cancers, making it a specific molecular marker and a potential therapeutic target for breast cancer. Secreted Mammaglobin protein has been detected in the sera of some breast cancer patients and not in sera of patients with other tumor types, indicating Mammaglobin as a serum marker for breast cancer.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Mammaglobin 1, Mammaglobin A, MGB1, SCGB2A2, UGB2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.