Human Methylmalonyl Coenzyme A mutase ELISA Kit
Cat no : KE00830
Synonyms
MUT, EC:5.4.99.2, MCM, Methylmalonyl CoA isomerase, Methylmalonyl-CoA isomerase
Validation Data Gallery
Product Information
KE00830 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Methylmalonyl Coenzyme A mutase ELISA kit is to be used to detect and quantify protein levels of endogenous human Methylmalonyl Coenzyme A mutase. The assay recognizes human Methylmalonyl Coenzyme A mutase. An antibody specific for human Methylmalonyl Coenzyme A mutase has been pre-coated onto the microwells. The human Methylmalonyl Coenzyme A mutase protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Methylmalonyl Coenzyme A mutase is added to detect the captured human Methylmalonyl Coenzyme A mutase protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Methylmalonyl Coenzyme A mutase ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4594 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 98% | 98%-99% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.03 | 0.14 | 2.73 |
| 2 | 8 | 1.34 | 0.07 | 4.87 |
| 3 | 8 | 0.73 | 0.06 | 7.83 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.34 | 0.42 | 7.85 |
| 2 | 16 | 1.32 | 0.05 | 3.96 |
| 3 | 16 | 0.72 | 0.04 | 6.09 |
Background Information
Methylmalonyl Coenzyme A mutase (MUT) is an enzyme that plays a crucial role in the metabolism of certain amino acids and fatty acids. Mutations in the MUT gene can lead to methylmalonic acidemia, a metabolic disorder characterized by the accumulation of toxic compounds such as methylmalonyl-CoA and propionyl-CoA, resulting in life-threatening metabolic acidosis, respiratory distress, neurological impairment, hyperammonemia, and ketosis. MUT is essential for maintaining normal metabolic processes and its dysfunction can have significant health implications, highlighting its importance in both basic metabolism and clinical medicine.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MUT, EC:5.4.99.2, MCM, Methylmalonyl CoA isomerase, Methylmalonyl-CoA isomerase |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.