Human/Mouse EHD2 ELISA Kit
Cat no : KE00985
Synonyms
EH domain containing 2, EH domain containing protein 2, EH domain-containing protein 2, PAST homolog 2, PAST2
Validation Data Gallery
Product Information
KE00985 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse EHD2 ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse EHD2. The assay recognizes human/mouse EHD2. An antibody specific for human/mouse EHD2 has been pre-coated onto the microwells. The human/mouse EHD2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse EHD2 is added to detect the captured human/mouse EHD2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse EHD2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.15 ng/mL |
| Range | 0.39-25 ng/mL |
| Reactivity | Human/Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 30846 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 115% | 108%-126% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 8 | 11.76 | 0.22 |
| 2 | 8 | 8 | 3.23 | 0.09 |
| 3 | 8 | 8 | 1.43 | 0.04 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 12.47 | 0.84 | 6.70 |
| 2 | 16 | 3.25 | 0.10 | 3.09 |
| 3 | 16 | 1.52 | 0.10 | 6.83 |
Background Information
EH domain-containing protein 2 (EHD2) is a member of the dynamin-related ATPase family that orchestrates membrane remodeling and vesicular trafficking at the plasma membrane. First identified as a caveolar-associated protein, EHD2 forms ATP-dependent oligomers that tether caveolae to the underlying actin cytoskeleton, thereby stabilizing these invaginated micro-domains and restraining their internalization. Beyond its membrane-sculpting role, EHD2 also shuttles to the nucleus where it functions as a transcriptional repressor, linking mechanical cues sensed at caveolae to gene-expression programs. In adipocytes, EHD2 is enriched at lipid-droplet contact sites and is required for insulin-dependent GLUT4 translocation, highlighting its importance for glucose homeostasis. Decreased EHD2 expression has been associated with poor prognosis in hepatocellular carcinoma and breast cancer, suggesting a tumor-suppressive function. Thus, EHD2 operates as a mechanotransducer that integrates membrane dynamics, metabolic signaling, and transcriptional control.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EH domain containing 2, EH domain containing protein 2, EH domain-containing protein 2, PAST homolog 2, PAST2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.