Human/Mouse Ezrin ELISA Kit
Cat no : KE00946
Synonyms
EZR, VIL2, Villin-2
Validation Data Gallery
Product Information
KE00946 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse Ezrin ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse Ezrin. The assay recognizes human/mouse Ezrin. An antibody specific for human/mouse Ezrin has been pre-coated onto the microwells. The human/mouse Ezrin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse Ezrin is added to detect the captured human/mouse Ezrin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse Ezrin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum, Human plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human/Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7430 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 88% | 81%-93% |
| Cell lysate | 103% | 97%-108% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.99 | 0.14 | 2.71 |
| 2 | 8 | 1.28 | 0.03 | 2.28 |
| 3 | 8 | 0.61 | 0.01 | 2.10 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.81 | 0.23 | 4.79 |
| 2 | 16 | 1.24 | 0.05 | 3.96 |
| 3 | 16 | 0.63 | 0.04 | 5.55 |
Background Information
Ezrin is a member of the ERM (Ezrin, Radixin, Moesin) protein family and serves as a crucial linker between the cell membrane and the actin cytoskeleton. It regulates cell morphology and movement by binding to transmembrane proteins (such as CD44 and EGFR) and actin filaments. Ezrin plays significant roles in various cellular processes, including maintaining cell morphology, facilitating cell movement and adhesion, regulating signal transduction, and influencing apoptosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | EZR, VIL2, Villin-2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.