Human/Mouse HCN1 ELISA Kit
Cat no : KE00852
Synonyms
BCNG-1, Brain cyclic nucleotide-gated channel 1, HAC 2, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
Validation Data Gallery
Product Information
KE00852 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse HCN1 ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse HCN1. The assay recognizes human/mouse HCN1. An antibody specific for human/mouse HCN1 has been pre-coated onto the microwells. The human/mouse HCN1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse HCN1 is added to detect the captured human/mouse HCN1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse HCN1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 1.1 pg/mL |
| Range | 23.4-1500 pg/mL |
| Reactivity | Human/Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 348980 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 86% | 79%-92% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 787.4 | 36.0 | 4.6 |
| 2 | 8 | 211.3 | 9.7 | 4.6 |
| 3 | 8 | 105.8 | 11.3 | 10.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 763.0 | 47.5 | 6.2 |
| 2 | 16 | 201.1 | 18.2 | 9.0 |
| 3 | 16 | 99.0 | 12.9 | 13.0 |
Background Information
HCN1 (Hyperpolarization-activated Cyclic Nucleotide-gated Channel 1) is a voltage-gated ion channel that plays a crucial role in regulating the electrical activity of neurons and cardiac cells. HCN1 is highly expressed in the brain and heart. In the brain, it is involved in regulating neuronal excitability, synaptic transmission, and rhythmogenesis. In the heart, it contributes to the intrinsic pacemaker activity of cardiomyocytes. It is a potential therapeutic target for various neurological and cardiac disorders.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BCNG-1, Brain cyclic nucleotide-gated channel 1, HAC 2, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.