Human/Mouse HGD ELISA Kit
Cat no : KE00801
Synonyms
AKU, EC:1.13.11.5, HGO, Homogentisate 1,2 dioxygenase, Homogentisate 1,2-dioxygenase
Validation Data Gallery
Product Information
KE00801 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human HGD ELISA kit is to be used to detect and quantify protein levels of endogenous human HGD. The assay recognizes human HGD. An antibody specific for human HGD has been pre-coated onto the microwells. The human HGD protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human HGD is added to detect the captured human HGD protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse HGD ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.07 ng/mL |
| Range | 0.78-50 ng/mL |
| Reactivity | Human/Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3081 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 105% | 102%-112% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 25.55 | 1.89 | 7.40 |
| 2 | 8 | 6.30 | 0.31 | 5.00 |
| 3 | 8 | 3.62 | 0.26 | 7.32 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 26.44 | 2.25 | 8.53 |
| 2 | 16 | 6.44 | 0.34 | 5.34 |
| 3 | 16 | 3.49 | 0.26 | 7.36 |
Background Information
Homogentisate1,2-dioxygenase (HGD), also named as HGO, is a mononuclear Fe(II)-dependent oxygenase that catalyzes the third step in the pathway for the catabolism of tyrosine, the conversion of homogentisate (HG) to maleylacetoacetate (MAA), and it can exsit as a dimer or trimer. HGD consists of a single type of subunit with no intermolecular disulfide bridges and requires Fe2+ as a cofactor. Deficiency of HGD leads to alkaptonuria(AKU), a rare genetic disorder characterized by the accumulation of homogentisic acid in connective tissue, resulting in ochronosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AKU, EC:1.13.11.5, HGO, Homogentisate 1,2 dioxygenase, Homogentisate 1,2-dioxygenase |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.