Human/Mouse LIMPII ELISA Kit
Cat no : KE01004
Synonyms
SCARB2, AMRF, CD36, CD36 antigen like 2, CD36 antigen-like 2
Validation Data Gallery
Product Information
KE01004 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse LIMPII ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse LIMPII. The assay recognizes human/mouse LIMPII. An antibody specific for human/mouse LIMPII has been pre-coated onto the microwells. The human/mouse LIMPII protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse LIMPII is added to detect the captured human/mouse LIMPII protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse LIMPII ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma,Cell lysates |
| Assay type | Sandwich |
| Sensitivity | 0.19 ng/mL |
| Range | 1.25-80 ng/mL |
| Reactivity | Human/Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 950 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 99% | 81%-126% |
| Cell lysate | 103% | 97%-123% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 33.07 | 1.93 | 5.84 |
| 2 | 8 | 7.53 | 0.63 | 8.31 |
| 3 | 8 | 4.03 | 0.31 | 7.61 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 33.74 | 2.77 | 8.22 |
| 2 | 16 | 7.71 | 0.70 | 9.11 |
| 3 | 16 | 4.27 | 0.38 | 8.89 |
Background Information
Lysosome membrane protein II (LIMPII) is a member of the CD36 family. It is a lysosomal membrane protein that plays a role in the targeting of lysosomal enzymes and the regulation of lysosomal function. LIMPII is involved in the transport and processing of lysosomal enzymes, ensuring their proper delivery to lysosomes. Additionally, LIMPII has been identified as a receptor for glucocerebrosidase (GCase), an enzyme involved in the degradation of glucocerebroside. This receptor function is crucial for the proper functioning of lysosomes and the overall metabolic health of the cell.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | SCARB2, AMRF, CD36, CD36 antigen like 2, CD36 antigen-like 2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.