Human/Mouse LMO7 ELISA Kit
Cat no : KE00617
Synonyms
FBX20, LOMP
Validation Data Gallery
Product Information
KE00617 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse LMO7 ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse LMO7. The assay recognizes human/mouse LMO7. An antibody specific for human/mouse LMO7 has been pre-coated onto the microwells. The human/mouse LMO7 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse LMO7 is added to detect the captured human/mouse LMO7 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse LMO7 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Lysate |
| Assay type | Sandwich |
| Sensitivity | 1.1 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human/Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4008 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 114% | 102%-125% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 897.0 | 43.5 | 4.9 |
| 2 | 8 | 226.8 | 8.5 | 3.7 |
| 3 | 8 | 114.9 | 4.7 | 4.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 943.5 | 86.8 | 9.2 |
| 2 | 16 | 238.9 | 18.6 | 7.8 |
| 3 | 16 | 121.1 | 8.7 | 7.2 |
Background Information
LMO7, also known as LOMP, FBX20, belongs to the single Calponin-homology (CH) domain-containing protein family, with a single actin-binding CH domain at the amino terminal end. LMO7 also contains a PDZ domain and a carboxyl-terminal LIM domain, both of which have been described as protein-protein interacting domains. LMO7 is involved in cell adhesion, cytoskeletal organization, and cellular signaling pathways, and dysregulation of LMO7 has been implicated in various malignancies.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FBX20, LOMP |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.