Human/Mouse LRRK2 ELISA Kit
Cat no : KE00500
Synonyms
LRRK2, AURA17, Dardarin, EC:2.7.11.1, leucine rich repeat kinase 2
Validation Data Gallery
Product Information
KE00500 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse LRRK2 ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse LRRK2. The assay recognizes human/mouse LRRK2. An antibody specific for human/mouse LRRK2 has been pre-coated onto the microwells. The human/mouse LRRK2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse LRRK2 is added to detect the captured human/mouse LRRK2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse LRRK2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.234-15 ng/mL |
| Reactivity | Human/Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 120892 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 99% | 93%-106% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 6.69 | 0.15 | 2.23 |
| 2 | 8 | 1.53 | 0.07 | 4.46 |
| 3 | 8 | 0.83 | 0.05 | 5.51 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 6.65 | 0.52 | 7.78 |
| 2 | 16 | 1.65 | 0.16 | 9.87 |
| 3 | 16 | 0.84 | 0.05 | 5.46 |
Background Information
Leucine-rich repeat kinase 2 (LRRK2) is a large multidomain protein belonging to the ROCO protein family. It contains several functional domains, including an ankyrin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a RAS domain, a GTPase domain, and a WD40 domain. LRRK2 is involved in various cellular processes, including synaptic vesicle recycling, vesicle sorting via the Golgi apparatus, regulation of lysosomal function, cytoskeleton dynamics, and immune system regulation. LRRK2 is best known for its role in Parkinson's disease (PD). Mutations in the LRRK2 gene typically enhance its kinase activity, leading to neurodegeneration.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | LRRK2, AURA17, Dardarin, EC:2.7.11.1, leucine rich repeat kinase 2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.