Human Leptin ELISA Kit
Cat no : KE00510
Synonyms
LEP, OB, Obese protein, Obesity factor, OBS
Validation Data Gallery
Product Information
KE00510 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Leptin ELISA kit is to be used to detect and quantify protein levels of endogenous human Leptin. The assay recognizes human Leptin. An antibody specific for human Leptin has been pre-coated onto the microwells. The human Leptin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Leptin is added to detect the captured human Leptin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Leptin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3952 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 102% | 94%-108% |
| Cell lysate | 97% | 94%-99% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 776.7 | 15.5 | 2.0 |
| 2 | 8 | 427.7 | 7.6 | 1.8 |
| 3 | 8 | 198.9 | 7.3 | 3.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 781.9 | 17.6 | 2.3 |
| 2 | 16 | 426.8 | 6.7 | 1.6 |
| 3 | 16 | 201.0 | 6.8 | 3.4 |
Background Information
Leptin is a 16 kDa adipocyte-derived hormone, and it has attracted interest in the field of obesity research due to its role in the regulation of energy balance. Leptin, secreted from adipose tissues in proportion to the fat store, acts in the hypothalamus to regulate feeding behavior. leptin has also been shown to play an essential role in tumorigenesis, tumor progression, and metastasis, due to its oncogenic, mitogenic, pro-inflammatory, and pro-angiogenic actions. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. Leptin has also been linked to type 2 diabetes mellitus development.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | LEP, OB, Obese protein, Obesity factor, OBS |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Clin Pediatr Endocrinol The serum oxytocin correlated with serum leptin and body mass index in Japanese adolescents. |