Human/Mouse PER1 ELISA Kit
Cat no : KE00743
Synonyms
hPER, hPER1, KIAA0482, PER, period homolog 1 (Drosophila)
Validation Data Gallery
Product Information
KE00743 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse PER1 ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse PER1. The assay recognizes human/mouse PER1. An antibody specific for human/mouse PER1 has been pre-coated onto the microwells. The human/mouse PER1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse PER1 is added to detect the captured human/mouse PER1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse PER1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 15.8 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human/Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5187 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 102% | 101%-105% |
| Tissue lysate | 89% | 84%-95% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 2,510.0 | 113.2 | 4.5 |
| 2 | 8 | 615.9 | 28.5 | 4.6 |
| 3 | 8 | 305.4 | 13.1 | 4.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,550.3 | 114.8 | 4.5 |
| 2 | 16 | 593.7 | 39.6 | 6.7 |
| 3 | 16 | 295.9 | 15.0 | 5.1 |
Background Information
PER1 (Period Circadian Clock 1) is a master regulator of circadian rhythm and functions in the nucleus to repress expression of the central circadian clock genes. The periodicity of PER1 abundance, nuclear translocation, and transcriptional repression is regulated by PER1 phosphorylation, ubiquitination, and proteasomal degradation. It is one component of the circadian clock mechanism which is essential for generating circadian rhythms. It can influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | hPER, hPER1, KIAA0482, PER, period homolog 1 (Drosophila) |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.