Human/Mouse/Rat HCN2 ELISA Kit
Cat no : KE00639
Synonyms
BCNG2, BCNG-2, Brain cyclic nucleotide-gated channel 2, HAC 1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
Validation Data Gallery
Product Information
KE00639 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse/rat HCN2 ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse/rat HCN2. The assay recognizes human/mouse/rat HCN2. An antibody specific for human/mouse/rat HCN2 has been pre-coated onto the microwells. The human/mouse/rat HCN2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse/rat HCN2 is added to detect the captured human/mouse/rat HCN2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse/Rat HCN2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 3.6 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human/Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 610 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue lysate | 106% | 100%-113% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 509.4 | 17.8 | 3.5 |
| 2 | 8 | 119.7 | 3.8 | 3.2 |
| 3 | 8 | 55.3 | 2.4 | 4.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 505.3 | 15.5 | 3.1 |
| 2 | 16 | 118.8 | 3.7 | 3.1 |
| 3 | 16 | 56.6 | 2.6 | 4.7 |
Background Information
Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are a group of voltage-gated ion channels in which the threshold voltage is modulated by the metabolic state of the cell (levels of cyclic nucleotides, like cAMP), thus allowing them to regulate cellular voltage in a context-specific manner. They open at hyperpolarized (negative) Vmem, giving rise to currents that are a mix of sodium and potassium fluxes. Among the four HCN isoforms (HCN1-4), HCN2 channels show an especially high sensitivity to cAMP.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BCNG2, BCNG-2, Brain cyclic nucleotide-gated channel 2, HAC 1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.