Human/Mouse/Rat HCN4 ELISA Kit
Cat no : KE00891
Synonyms
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
Validation Data Gallery
Product Information
KE00891 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse/rat HCN4 ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse/rat HCN4. The assay recognizes human/mouse/rat HCN4. An antibody specific for human/mouse/rat HCN4 has been pre-coated onto the microwells. The human/mouse/rat HCN4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse/rat HCN4 is added to detect the captured human/mouse/rat HCN4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse/Rat HCN4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 7.1 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human/Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10021 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 96% | 81%-115% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 482.6 | 13.7 | 2.8 |
| 2 | 8 | 122.1 | 3.0 | 2.5 |
| 3 | 8 | 64.7 | 1.1 | 1.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 498.6 | 25.2 | 5.1 |
| 2 | 16 | 123.7 | 4.5 | 3.6 |
| 3 | 16 | 63.2 | 3.0 | 4.7 |
Background Information
HCN4 (Hyperpolarization-activated Cyclic Nucleotide-gated Channel 4) belongs to the potassium channel HCN family. It is a hyperpolarization-activated ion channel with very slow activation and inactivation, exhibiting weak selectivity for potassium over sodium ions. HCN4 may contribute to the native pacemaker currents in the heart (If) and in neurons (Ih). It is regulated by cyclic nucleotides such as cAMP, which modulate channel activity by shifting the voltage dependence of activation and altering the rate of channel opening and closing. HCN4 may mediate responses to sour stimuli. Defects in HCN4 are a cause of sick sinus syndrome type 2 (SSS2).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.