Human/Mouse/Rat MAP2 ELISA Kit
Cat no : KE00917
Synonyms
MAP 2, MAP-2, MAP2A, MAP2B, MAP2C
Validation Data Gallery
Product Information
KE00917 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse/rat MAP2 ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse/rat MAP2. The assay recognizes human/mouse/rat MAP2. An antibody specific for human/mouse/rat MAP2 has been pre-coated onto the microwells. The human/mouse/rat MAP2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse/rat MAP2 is added to detect the captured human/mouse/rat MAP2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse/Rat MAP2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum, Human plasma, Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 11.0 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Human/Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4133 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 109% | 102%-126% |
| Lysate | 101% | 84%-128% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,351.2 | 30.6 | 2.3 |
| 2 | 8 | 435.0 | 20.3 | 4.7 |
| 3 | 8 | 172.1 | 5.9 | 3.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,334.1 | 42.5 | 3.2 |
| 2 | 16 | 436.6 | 16.0 | 3.7 |
| 3 | 16 | 177.2 | 11.0 | 6.2 |
Background Information
Microtubule-associated protein 2 (MAP2) is a tubulin binding protein regulating the spacing and stability of microtubules and contributing to elongation of dendrites.Multiple high molecular weight and low molecular weight MAP2 isoforms are expressed within axons, dendrites, and cell bodies. MAP2 isoforms differ in their tissue and developmental expression pattern. In the brain, MAP2B is widely expressed during and post development, MAP2A is expressed postnatally, while MAP2C is present only in the early development except of present in photosensitive cells of the adult retina and in the olfactory system. MAP proteins are highly expressed in the CNS found in cell bodies and dendrites of neurons, in dorsal root ganglion, reactive glia, and in the testis. In neurons, MAP2 proteins are found in the cell body and dendrites, where they associate with microtubules, while they can also be present in the nuclei of testicular cells. MAP2 proteins are abundantly expressed in neurons. MAP2 is frequently used as a dendritic marker because it is present in the cell body and dendrites of neurons but absent in axons.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MAP 2, MAP-2, MAP2A, MAP2B, MAP2C |