Human/Mouse/Rat SHC1 ELISA Kit
Cat no : KE00764
Synonyms
SHC, SH2 domain protein C1, SHC transforming protein 1, SHC-transforming protein 1, SHC-transforming protein 3
Validation Data Gallery
Product Information
KE00764 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse/rat SHC1 ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse/rat SHC1. The assay recognizes human/mouse/rat SHC1. An antibody specific for human/mouse/rat SHC1 has been pre-coated onto the microwells. The human/mouse/rat SHC1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse/rat SHC1 is added to detect the captured human/mouse/rat SHC1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse/Rat SHC1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human/Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6464 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 92% | 83%-100% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.44 | 0.21 | 4.75 |
| 2 | 8 | 1.02 | 0.05 | 5.28 |
| 3 | 8 | 0.55 | 0.02 | 2.96 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.30 | 0.23 | 5.35 |
| 2 | 16 | 1.04 | 0.05 | 5.00 |
| 3 | 16 | 0.53 | 0.03 | 5.62 |
Background Information
SHC1 (Src homology 2 domain containing transforming protein 1) is a member of the SHC family, which includes SHCA, SHCB, SHCC, and SHCD4. The SHC1 gene is located on human chromosome 1 and encodes a protein involved in various cellular processes. SHC1 is believed to play a role in inhibiting neuronal apoptosis and regulating the receptor tyrosine kinase pathway. It has also been implicated in cancer development and progression, with studies highlighting its potential as a diagnostic, prognostic, and immunological biomarker in various cancers. For example, SHC1 is highly expressed in multiple cancer types and is associated with worse prognosis. Additionally, SHC1 gene mutations have been linked to poor overall survival and disease-free survival in certain cancers.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | SHC, SH2 domain protein C1, SHC transforming protein 1, SHC-transforming protein 1, SHC-transforming protein 3 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.