Human/Mouse/Rat TrkB ELISA Kit
Cat no : KE01011
Synonyms
NTRK2, BDNF/NT-3 growth factors receptor, GP145 TrkB, GP145-TrkB, NTRK 2
Validation Data Gallery
Product Information
KE01011 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse/rat TrkB ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse/rat TrkB. The assay recognizes human/mouse/rat TrkB. An antibody specific for human/mouse/rat TrkB has been pre-coated onto the microwells. The human/mouse/rat TrkB protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse/rat TrkB is added to detect the captured human/mouse/rat TrkB protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse/Rat TrkB ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.27 ng/mL |
| Range | 1.56-100 ng/mL |
| Reactivity | Human/Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4915 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 89% | 80%-97% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 52.10 | 1.97 | 3.78 |
| 2 | 8 | 10.80 | 0.47 | 4.36 |
| 3 | 8 | 5.38 | 0.41 | 7.55 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 54.72 | 6.72 | 12.28 |
| 2 | 16 | 11.06 | 1.01 | 9.13 |
| 3 | 16 | 5.14 | 0.42 | 8.27 |
Background Information
Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance through regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | NTRK2, BDNF/NT-3 growth factors receptor, GP145 TrkB, GP145-TrkB, NTRK 2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.