Human/Mouse TOLLIP ELISA Kit
Cat no : KE00811
Synonyms
IL 1RAcPIP, toll interacting protein, Toll-interacting protein
Validation Data Gallery
Product Information
KE00811 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse TOLLIP ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse TOLLIP. The assay recognizes human/mouse TOLLIP. An antibody specific for human/mouse TOLLIP has been pre-coated onto the microwells. The human/mouse TOLLIP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse TOLLIP is added to detect the captured human/mouse TOLLIP protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse TOLLIP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.39-25 ng/mL |
| Reactivity | Human/Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 54472 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 87% | 80%-97% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 11.43 | 0.64 | 5.58 |
| 2 | 8 | 3.38 | 0.13 | 3.77 |
| 3 | 8 | 1.91 | 0.07 | 3.55 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 11.60 | 0.78 | 6.75 |
| 2 | 16 | 3.26 | 0.18 | 5.57 |
| 3 | 16 | 1.84 | 0.12 | 6.57 |
Background Information
TOLLIP (Toll-interacting protein) is an intracellular adaptor protein with diverse actions throughout the body. In a context- and cell type-specific manner, TOLLIP can function as an inhibitor of inflammation and endoplasmic-reticulum stress, an activator of autophagy, or a critical regulator of intracellular vacuole trafficking. TOLLIP plays a central role in autophagy, in which it binds with the central protein LC3, which complexes with ubiquitinated protein aggregates that are then shuttled via autophagosomes to lysosomes for clearance.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL 1RAcPIP, toll interacting protein, Toll-interacting protein |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.