Human/Mouse TRPV4 ELISA Kit
Cat no : KE00624
Synonyms
Osm 9 like TRP channel 4, Osm-9-like TRP channel 4, OTRPC4, TRP12, Vanilloid receptor-like channel 2
Validation Data Gallery
Product Information
KE00624 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse TRPV4 ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse TRPV4. The assay recognizes human/mouse TRPV4. An antibody specific for human/mouse TRPV4 has been pre-coated onto the microwells. The human/mouse TRPV4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse TRPV4 is added to detect the captured human/mouse TRPV4 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse TRPV4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.5 pg/mL |
| Range | 19.5-1250 pg/mL |
| Reactivity | Human/Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 59341 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 101% | 89%-105% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 650.0 | 16.2 | 2.5 |
| 2 | 8 | 167.3 | 2.2 | 1.3 |
| 3 | 8 | 82.6 | 2.4 | 2.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 626.1 | 32.9 | 5.2 |
| 2 | 16 | 167.5 | 2.6 | 1.5 |
| 3 | 16 | 79.6 | 6.1 | 7.7 |
Background Information
TRPV4, also named as VRL2 and VROAC, belongs to the transient receptor family and TrpV subfamily. It is a non-selective calcium permeant cation channel protein which involved in osmotic sensitivity and mechanosensitivity. It is activated by low pH, citrate and phorbol esters. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Defects in TRPV4 are the cause of brachyolmia type 3 (BRAC3), spondylometaphyseal dysplasia Kozlowski type (SMDK) and metatropic dysplasia (MTD). Multiple transcript variants encoding different isoforms have been found.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Osm 9 like TRP channel 4, Osm-9-like TRP channel 4, OTRPC4, TRP12, Vanilloid receptor-like channel 2 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.