Human/Mouse WASP ELISA Kit
Cat no : KE00733
Synonyms
Actin nucleation-promoting factor WAS, WAS, Wiskott-Aldrich syndrome protein
Validation Data Gallery
Product Information
KE00733 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse WASP ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse WASP. The assay recognizes human/mouse WASP. An antibody specific for human/mouse WASP has been pre-coated onto the microwells. The human/mouse WASP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse WASP is added to detect the captured human/mouse WASP protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse WASP ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 18.1 pg/mL |
| Range | 125-8000 pg/mL |
| Reactivity | Human/Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7454 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Lysate | 85% | 81%-89% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 6,404.4 | 292.5 | 4.6 |
| 2 | 8 | 1,674.7 | 31.0 | 1.9 |
| 3 | 8 | 777.9 | 15.9 | 2.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 6,553.7 | 287.2 | 4.4 |
| 2 | 16 | 1,597.1 | 92.1 | 5.8 |
| 3 | 16 | 793.6 | 23.5 | 3.0 |
Background Information
WASP (Wiskott-Aldrich Syndrome Protein) is a key regulator of the actin cytoskeleton and plays a crucial role in various cellular processes, including cell movement, signal transduction, and immune response. Essential for binding to the Arp2/3 complex, which promotes actin polymerization.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Actin nucleation-promoting factor WAS, WAS, Wiskott-Aldrich syndrome protein |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.