Human Myoglobin ELISA Kit
Cat no : KE00204
Synonyms
MB, myoglobin, PVALB
Validation Data Gallery
Product Information
KE00204 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Myoglobin ELISA kit is to be used to detect and quantify protein levels of endogenous Myoglobin. The assay recognizes human Myoglobin. An antibody specific for Myoglobin has been pre-coated onto the microwells. The Myoglobin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for Myoglobin is added to detect the captured Myoglobin protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Myoglobin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.24 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4151 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 97% | 82%-115% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 18.3 | 1.2 | 6.6 |
| 2 | 20 | 5.0 | 0.4 | 8.0 |
| 3 | 20 | 1.2 | 0.1 | 8.3 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 23.6 | 2.2 | 9.3 |
| 2 | 24 | 6.6 | 0.6 | 9.1 |
| 3 | 24 | 1.2 | 0.1 | 8.3 |
Background Information
Myoglobin is a cytoplasmic hemoprotein that is expressed primarily in cardiomyocytes and oxidative skeletal muscle fibers, functioning on facilitating oxygen transport and modulating nitric oxide homeostasis within cardiac and skeletal myocytes. Elevated serum levels of myoglobin are found in the bloodstream after muscle injury. Serum myoglobin is one of the earliest markers for the diagnosis of acute myocardial infarction.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MB, myoglobin, PVALB |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.