Human NCR1 ELISA Kit
Cat no : KE00980
Synonyms
CD335, hNKp46, LY94, Natural killer cell p46-related protein, NCR 1
Validation Data Gallery
Product Information
KE00980 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human NCR1 ELISA kit is to be used to detect and quantify protein levels of endogenous human NCR1. The assay recognizes human NCR1. An antibody specific for human NCR1 has been pre-coated onto the microwells. The human NCR1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human NCR1 is added to detect the captured human NCR1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human NCR1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 3.8 pg/mL |
| Range | 39-2500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9437 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 84% | 77%-94% |
| Cell culture supernatant | 102% | 99%-107% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,242.2 | 53.6 | 4.3 |
| 2 | 8 | 295.7 | 5.9 | 2.0 |
| 3 | 8 | 157.5 | 3.0 | 1.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,232.1 | 60.9 | 4.9 |
| 2 | 16 | 307.9 | 15.3 | 5.0 |
| 3 | 16 | 164.6 | 9.0 | 5.4 |
Background Information
NKp46, also known as NCR1 or CD335, is an Ig-like superfamily cell surface receptor that is highly conserved in mammals. NKp46 is a type I transmembrane glycoprotein consisting of two extracellular Ig-like domains, a transmembrane domain, and an intracellular tail. It is expressed on NK cells, rare T-cell subsets, and a mucosal population of NKp46+ innate lymphoid cells. NKp46 is the major triggering receptor involved in natural cytotoxicity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD335, hNKp46, LY94, Natural killer cell p46-related protein, NCR 1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.