Human NDUFB8 ELISA Kit
Cat no : KE00986
Synonyms
ASHI, CI ASHI, CIASHI, CI-ASHI, Complex I-ASHI
Validation Data Gallery
Product Information
KE00986 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human NDUFB8 ELISA kit is to be used to detect and quantify protein levels of endogenous human NDUFB8. The assay recognizes human NDUFB8. An antibody specific for human NDUFB8 has been pre-coated onto the microwells. The human NDUFB8 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human NDUFB8 is added to detect the captured human NDUFB8 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human NDUFB8 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 9.4 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4714 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 84% | 78%-89% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,991.9 | 42.7 | 2.1 |
| 2 | 8 | 495.0 | 17.4 | 3.5 |
| 3 | 8 | 251.4 | 8.9 | 3.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 2,053.3 | 94.8 | 4.6 |
| 2 | 16 | 507.8 | 22.1 | 4.4 |
| 3 | 16 | 262.1 | 13.1 | 5.0 |
Background Information
NDUFS8 is encoded by a nuclear gene and is a member of the respiratory chain complex I. Respiratory chain complex I (CI; NADH:ubiquinone oxidoreductase), one of the largest membrane-bound protein complexes in the human cell, is essential for oxidative energy metabolism as it powers ATP synthesis by using the reducing potential of NADH to proton translocation across the inner mitochondrial membrane.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ASHI, CI ASHI, CIASHI, CI-ASHI, Complex I-ASHI |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.