Human NEIL1 ELISA Kit
Cat no : KE00878
Synonyms
DNA-(apurinic or apyrimidinic site) lyase Neil1, EC:3.2.2.-, Endonuclease 8-like 1, Endonuclease VIII-like 1, NEI L1
Validation Data Gallery
Product Information
KE00878 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human NEIL1 ELISA kit is to be used to detect and quantify protein levels of endogenous human NEIL1. The assay recognizes human NEIL1. An antibody specific for human NEIL1 has been pre-coated onto the microwells. The human NEIL1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human NEIL1 is added to detect the captured human NEIL1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human NEIL1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.26 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 79661 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 76% | 71%-84% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 17.55 | 1.31 | 7.46 |
| 2 | 8 | 6.30 | 0.29 | 4.57 |
| 3 | 8 | 2.14 | 0.17 | 7.93 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 17.93 | 1.30 | 7.24 |
| 2 | 16 | 6.27 | 0.28 | 4.54 |
| 3 | 16 | 2.21 | 0.20 | 8.84 |
Background Information
NEIL1 is a versatile DNA repair enzyme as it has a broad range and overlapping substrate specificity with other DNA glycosylases. NEIL1 can efficiently recognize and remove 5-hydroxyuracil, 5-hydroxycytosine, thymine glycol, and formamidopyrimidine (FAPY) lesions. NEIL1 also participates in interstrand cross-link repair and nucleotide excision repair in addition to BER.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | DNA-(apurinic or apyrimidinic site) lyase Neil1, EC:3.2.2.-, Endonuclease 8-like 1, Endonuclease VIII-like 1, NEI L1 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.